Nitroalkane oxidase

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nitroethane oxidase

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In enzymology, a nitroalkane oxidase (EC 1.7.3.1) is an enzyme that catalyzes the chemical reaction

a nitroalkane + H₂O + O₂

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an aldehyde or ketone + nitrite + H₂O₂

The 3 substrates of this enzyme are nitroalkane, H₂O, and O₂, whereas its 4 products are aldehyde, ketone, nitrite, and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with oxygen as acceptor. The systematic name of this enzyme class is nitroalkane:oxygen oxidoreductase. Other names in common use include nitroethane oxidase, NAO, and nitroethane:oxygen oxidoreductase. This enzyme participates in nitrogen metabolism.

References

LITTLE HN (1951). "Oxidation of nitroethane by extracts from Neurospora". J. Biol. Chem. 193 (1): 347–58. doi:10.1016/S0021-9258(19)52460-7. PMID 14907722.
Kido T, Hashizume K, Soda K (1978). "Purification and properties of nitroalkane oxidase from Fusarium oxysporum". J. Bacteriol. 133 (1): 53–8. doi:10.1128/jb.133.1.53-58.1978. PMC 221975. PMID 22538.
Daubner SC, Gadda G, Valley MP, Fitzpatrick PF (2002). "Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2702–7. doi:10.1073/pnas.052527799. PMC 122411. PMID 11867731.
Fitzpatrick PF, Orville AM, Nagpal A, Valley MP (2005). "Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase". Arch. Biochem. Biophys. 433 (1): 157–65. doi:10.1016/j.abb.2004.08.021. PMID 15581574.
Valley MP, Tichy SE, Fitzpatrick PF (2005). "Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase". J. Am. Chem. Soc. 127 (7): 2062–6. doi:10.1021/ja043542f. PMC 1630679. PMID 15713081.

v t e Oxidoreductases: nitrogenous donor (EC 1.7)
1.7.1	GMP reductase
1.7.2	Nitrite reductase (NO-forming)
1.7.3	Urate oxidase
1.7.7	Ferredoxin—nitrite reductase
1.7.99	Hydroxylamine reductase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

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