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  1. World Encyclopedia
  2. Nucleic acid methods - Wikipedia
Nucleic acid methods - Wikipedia
From Wikipedia, the free encyclopedia
Techniques used to study nucleic acids
For protein methods, see Protein methods.

Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

Purification

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  • DNA extraction[1]
  • Phenol–chloroform extraction
  • Minicolumn purification
  • RNA extraction
  • Boom method
  • Synchronous coefficient of drag alteration (SCODA) DNA purification

Quantification

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  • Abundance in weight: spectroscopic nucleic acid quantitation
  • Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
  • High-throughput relative abundance: DNA microarray
  • High-throughput absolute abundance: serial analysis of gene expression (SAGE)
  • Size: gel electrophoresis

Synthesis

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  • De novo: oligonucleotide synthesis
  • Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)

Kinetics

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  • Multi-parametric surface plasmon resonance[2][3]
  • Dual-polarization interferometry[4]
  • Quartz crystal microbalance with dissipation monitoring (QCM-D)[5]

Gene function

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  • RNA interference[6]

Other

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  • Bisulfite sequencing
  • DNA sequencing
  • Expression cloning
  • Fluorescence in situ hybridization
  • Lab-on-a-chip
  • Comparison of nucleic acid simulation software
  • Northern blot
  • Nuclear run-on assay
  • Radioactivity in the life sciences
  • Southern blot
  • Differential centrifugation (sucrose gradient)
  • Toeprinting assay
  • Several bioinformatics methods, as seen in list of RNA structure prediction software

See also

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  • CSH Protocols
  • Current Protocols

References

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  1. ^ Rana, Ajay K. (2025). "Challenging biological samples and strategies for DNA extraction". Journal of Investigative Medicine. 73 (6): 443–459. doi:10.1177/10815589251327503. PMID 40033560.
  2. ^ Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K. PMID 25295484.
  3. ^ Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology. 25 (9) 094009. Bibcode:2014Nanot..25i4009I. doi:10.1088/0957-4484/25/9/094009. PMID 24522116. S2CID 206070778.
  4. ^ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics. 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
  5. ^ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques. 19 (3): 151–158. PMC 2563918. PMID 19137101.
  6. ^ Hannon, Gregory J. (July 2002). "RNA interference". Nature. 418 (6894): 244–251. Bibcode:2002Natur.418..244H. doi:10.1038/418244a. ISSN 1476-4687. PMID 12110901. S2CID 4426281.

External links

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  • Protocols for Recombinant DNA Isolation, Cloning, and Sequencing
  • v
  • t
  • e
Molecular biology
  • History
  • Index
  • Glossary
Overview
Central dogma
  • DNA replication (DNA)
  • Transcription (RNA)
  • Translation (protein)
Element
    • Genetic
    • Heredity
  • Promoter
    • Pribnow box
    • TATA box
  • Operon
    • gal operon
    • lac operon
    • trp operon
  • Intron
  • Exon
  • Terminator
  • Enhancer
  • Repressor
    • lac repressor
    • trp repressor
  • Silencer
  • Histone methylation
Linked life
  • Cell biology
  • Biochemistry
  • Computational biology
  • Developmental biology
  • Functional biology/medicine
  • Genetics
Engineering
Concepts
  • Cultured meat
  • Mitosis
  • Cell signalling
  • Post-transcriptional modification
  • Post-translational modification
  • Dry lab / Wet lab
Techniques
  • Cell culture
  • Model organisms (such as C57BL/6 mice)
  • Methods
    • Nucleic acid
    • Protein
  • Fluorescence, Pigment & Radioactivity
High-throughput technique ("-omics")
DNA microarray
Mass spectrometry
Lab-on-a-chip
Gene regulation
  • Epigenetic
  • Genetic
  • Post-transcriptional
  • Post-translational regulation
  • Molecular biology
  • WikiProject
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