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TOP2A - Wikipedia

From Wikipedia, the free encyclopedia

TOP2A

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
1ZXM, 1ZXN, 4FM9, 4R1F

Identifiers

Aliases

TOP2A, TOP2, TP2A, topoisomerase (DNA) II alpha, DNA topoisomerase II alpha, TOPIIA, TOP2alpha

External IDs

OMIM: 126430; MGI: 98790; HomoloGene: 830; GeneCards: TOP2A; OMA:TOP2A - orthologs

Gene location (Human)

Chr.	Chromosome 17 (human)^[1]

Band	17q21.2	Start	40,388,525 bp^[1]
Band	17q21.2	End	40,417,896 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 11 (mouse)^[2]

Band	11 D\|11 62.91 cM	Start	98,883,769 bp^[2]
Band	11 D\|11 62.91 cM	End	98,915,015 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

ventricular zone

ganglionic eminence

secondary oocyte

right testis

left testis

bone marrow

trabecular bone

gonad

bone marrow cells

testicle

Top expressed in

genital tubercle

tail of embryo

ventricular zone

fetal liver hematopoietic progenitor cell

maxillary prominence

primitive streak

mandibular prominence

epiblast

tibiofemoral joint

somite

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	DNA binding nucleotide binding ATP-dependent activity, acting on DNA protein homodimerization activity histone deacetylase binding chromatin binding isomerase activity metal ion binding DNA topoisomerase activity protein C-terminus binding ubiquitin binding protein binding protein heterodimerization activity enzyme binding ATP binding protein kinase C binding magnesium ion binding DNA binding, bending RNA binding DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
Cellular component	cytoplasm DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex nucleoplasm nuclear chromosome nucleolus centriole condensed chromosome nucleus viral integration complex protein-containing complex ribonucleoprotein complex
Biological process	resolution of meiotic recombination intermediates apoptotic chromosome condensation embryonic cleavage rhythmic process chromosome segregation DNA metabolic process protein sumoylation cellular response to DNA damage stimulus DNA ligation chromosome condensation regulation of circadian rhythm hematopoietic progenitor cell differentiation positive regulation of apoptotic process mitotic DNA integrity checkpoint signaling positive regulation of viral genome replication positive regulation of single stranded viral RNA replication via double stranded DNA intermediate sister chromatid segregation positive regulation of transcription by RNA polymerase II DNA topological change negative regulation of DNA duplex unwinding female meiotic nuclear division
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


7153


21973

Ensembl


ENSG00000131747


ENSMUSG00000020914

UniProt


P11388


Q01320

RefSeq (mRNA)


NM_001067


NM_011623

RefSeq (protein)


NP_001058


NP_035753

Location (UCSC)

Chr 17: 40.39 – 40.42 Mb

Chr 11: 98.88 – 98.92 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

DNA topoisomerase IIα is a human enzyme encoded by the TOP2A gene.

Topoisomerase IIα relieves topological DNA stress during transcription, condenses chromosomes, and separates chromatids. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several chemotherapy agents^[5] and a variety of mutations in this gene have been associated with the development of drug resistance.^{[citation needed]} Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.^[6]

Interactions

TOP2A has been shown to interact with SMURF2,^[7] HDAC1,^[8]^[9] CDC5L,^[10] Small ubiquitin-related modifier 1,^[11] P53,^[12] and TOPBP1.^[13]

In other species

In Drosophila Hadlaczky et al 1988 found DNA topoisomerase II α to correlate with cell proliferation - but β did not.^[14]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000131747 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020914 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Deweese, J. E.; Osheroff, N. (2009-02-01). "The DNA cleavage reaction of topoisomerase II: wolf in sheep's clothing". Nucleic Acids Research. 37 (3): 738–748. doi:10.1093/nar/gkn937. ISSN 0305-1048. PMC 2647315. PMID 19042970.
^ "Entrez Gene: TOP2A topoisomerase (DNA) II alpha 170kDa".
^ Emanuelli A, Borroni AP, Apel-Sarid L, Shah PA, Ayyathan DM, Koganti P, et al. (August 2017). "Smurf2-Mediated Stabilization of DNA Topoisomerase IIα Controls Genomic Integrity". Cancer Research. 77 (16): 4217–4227. doi:10.1158/0008-5472.CAN-16-2828. PMID 28611047.
^ Tsai SC, Valkov N, Yang WM, Gump J, Sullivan D, Seto E (November 2000). "Histone deacetylase interacts directly with DNA topoisomerase II". Nature Genetics. 26 (3): 349–353. doi:10.1038/81671. PMID 11062478. S2CID 19301396.
^ Johnson CA, Padget K, Austin CA, Turner BM (February 2001). "Deacetylase activity associates with topoisomerase II and is necessary for etoposide-induced apoptosis". The Journal of Biological Chemistry. 276 (7): 4539–4542. doi:10.1074/jbc.C000824200. PMID 11136718.
^ Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (December 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–6581. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
^ Mao Y, Desai SD, Liu LF (August 2000). "SUMO-1 conjugation to human DNA topoisomerase II isozymes". The Journal of Biological Chemistry. 275 (34): 26066–26073. doi:10.1074/jbc.M001831200. PMID 10862613.
^ Cowell IG, Okorokov AL, Cutts SA, Padget K, Bell M, Milner J, Austin CA (February 2000). "Human topoisomerase IIalpha and IIbeta interact with the C-terminal region of p53". Experimental Cell Research. 255 (1): 86–94. doi:10.1006/excr.1999.4772. PMID 10666337.
^ Broderick, Ronan; Niedzwiedz, Wojciech (2015-08-12). "Sister chromatid decatenation: bridging the gaps in our knowledge". Cell Cycle. 14 (19): 3040–3044. doi:10.1080/15384101.2015.1078039. ISSN 1538-4101. PMC 4825568. PMID 26266709.
^ Wang JC (1996). "DNA topoisomerases". Annual Review of Biochemistry. 65 (1). Annual Reviews: 635–692. doi:10.1146/annurev.bi.65.070196.003223. PMID 8811192.

Interactions

In other species

See also

References

Further reading