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BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi.^[1] It was first described by A.J.P. Oort [Wikidata] in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981.^[1] The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.^[2]

Typical composition

BAF agar typically contains:^[2]

30.0 g/L glucose
2.0 g/L peptone
0.2 g/L yeast extract
0.5 g/L KH₂PO₄
0.5 g/L MgSO₄.7 H₂O
10.0 mg/L FeCl₃.6 H₂O
1.0 mg/L ZnSO₄.7 H₂O
5.0 mg/L MnSO₄
100.0 mg/L CaCl₂.2 H₂O
50.0 μg/L thiamine HCl
1.0 μg/L biotin
100.0 μg/L folic acid
50.0 μg/L inositol
15 g/L agar

References

^ ^a ^b Águeda, Beatriz; Parladé, Javier; Fernández-Toirán, Luz Marina; Cisneros, Óscar; de Miguel, Ana María; Modrego, María Pilar; Martínez-Peña, Fernando; Pera, Joan (October 2008). "Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)". Mycorrhiza. 18 (8): 443–449. Bibcode:2008Mycor..18..443A. doi:10.1007/s00572-008-0192-3. ISSN 0940-6360. PMID 18695982. S2CID 11233267.
^ ^a ^b "CBS List of Media" (PDF).

Growth media / agar plates

Selective media

Gram positive

Actinomycetota	Mycobacterium tuberculosis Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar Mycoplasma pneumoniae Eaton's agar
Bacillota	Corynebacterium diphtheriae Hoyle's agar Enterococcus Bile esculin agar Lactobacillus Lee's Agar MRS agar Lactococcus M17 agar Listeria Fraser broth PALCAM Staphylococcus Mannitol salt agar Baird-Parker agar Vogel–Johnson agar

Gram negative

Alphaproteobacteria	Brucella Brucella agar Farrell's medium
Betaproteobacteria	Neisseria Thayer–Martin agar New York City agar
Gammaproteobacteria	Aggregatibacter actinomycetemcomitans Tryptic soy-serum-bacitracin-vancomycin Bordetella Bordet–Gengou agar Enterobacteriaceae VRBD agar Haemophilus influenzae/Legionella pneumophila Buffered charcoal yeast extract agar Pseudomonas aeruginosa Cetrimide agar Salmonella XLT agar DCA agar Salmonella/Shigella XLD agar

Differential media

Fungal media

Nonselective media

Other/ungrouped media

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