Vogel–Johnson agar

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Vogel–Johnson agar is a type of agar growth medium selective for coagulase-positive staphylococci. It is used to isolate Staphylococcus aureus from clinical specimens and food. It was first described by Vogel and Johnson, who modified the Tellurite Glycine Agar recipe by Zebovitz et al. by doubling the mannitol concentration to 1% (w/v) and adding Phenol red as a pH indicator.^[1] It is widely available commercially.

Typical composition

Vogel–Johnson agar typically contains:^[2]

Tryptone - 10.0 g/L
Yeast extract - 5.0 g/L
Mannitol - 10.0 g/L
Dipotassium phosphate - 5.0 g/L
Lithium chloride - 5.0 g/L
Glycine - 10.0 g/L
Phenol red - 0.025 g/L
Agar = 16.0 g/L

The Modified Vogel-Johnson Agar contains, in addition to above: 5 g/L of beef extract, 2 g/L of deoxyribonucleic acid, 2 /L g of phosphatidyl choline and 780 units/L of catalase spread on the plates before inoculation.^[3]

References

^ Vogel R. A. and Johnson M. J. (1961) Pub. Hlth Lab. 18. 131.
^ Nutrition, Center for Food Safety and Applied (2020-06-26). "BAM Media M176: Vogel-Johnson (VJ) Agar". FDA.
^ Andrews, Gail P.; Martin, Scott E. (1978-07-01). "Modified Vogel and Johnson Agar for Staphylococcus aureus". Journal of Food Protection. 41 (7): 530–532. doi:10.4315/0362-028X-41.7.530. ISSN 0362-028X. PMID 30795108.

Growth media / agar plates

Selective media

Gram positive

Actinomycetota	Mycobacterium tuberculosis Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar Mycoplasma pneumoniae Eaton's agar
Bacillota	Corynebacterium diphtheriae Hoyle's agar Enterococcus Bile esculin agar Lactobacillus Lee's Agar MRS agar Lactococcus M17 agar Listeria Fraser broth PALCAM Staphylococcus Mannitol salt agar Baird-Parker agar Vogel–Johnson agar

Gram negative

Alphaproteobacteria	Brucella Brucella agar Farrell's medium
Betaproteobacteria	Neisseria Thayer–Martin agar New York City agar
Gammaproteobacteria	Aggregatibacter actinomycetemcomitans Tryptic soy-serum-bacitracin-vancomycin Bordetella Bordet–Gengou agar Enterobacteriaceae VRBD agar Haemophilus influenzae/Legionella pneumophila Buffered charcoal yeast extract agar Pseudomonas aeruginosa Cetrimide agar Salmonella XLT agar DCA agar Salmonella/Shigella XLD agar

Differential media

Fungal media

Nonselective media

Other/ungrouped media

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