Ent-kaurene oxidase

From Wikipedia, the free encyclopedia

Ent-kaurene oxidase

Identifiers

Databases

PDB structures

Ent-kaurene oxidase (EC 1.14.14.86, Formerly EC 1.14.13.78) is an enzyme with systematic name ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

ent-kaur-16-ene + 3 NADPH + 3 H⁺ + 3 O₂

\rightleftharpoons

ent-kaur-16-en-19-oate + 3 NADP⁺ + 4 H₂O (overall reaction)

(1a) ent-kaur-16-ene + NADPH + H⁺ + O₂

\rightleftharpoons

ent-kaur-16-en-19-ol + NADP⁺ + H₂O

(1b) ent-kaur-16-en-19-ol + NADPH + H⁺ + O₂

\rightleftharpoons

ent-kaur-16-en-19-al + NADP⁺ + 2 H₂O

(1c) ent-kaur-16-en-19-al + NADPH + O₂

\rightleftharpoons

ent-kaur-16-en-19-oate + NADP⁺ + H₂O

Ent-kaurene oxidase requires cytochrome P450 and is inhibited by paclobutrazol.^[4]

References

^ Ashman PJ, Mackenzie A, Bramley PM (November 1990). "Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi". Biochimica et Biophysica Acta (BBA) - General Subjects. 1036 (2): 151–7. doi:10.1016/0304-4165(90)90027-t. PMID 2223832.
^ Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM (May 1992). "Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima". Biochemical Society Transactions. 20 (2): 218S. doi:10.1042/bst020218s. PMID 1397591.
^ Helliwell CA, Poole A, Peacock WJ, Dennis ES (February 1999). "Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis". Plant Physiology. 119 (2): 507–10. doi:10.1104/pp.119.2.507. PMC 32127. PMID 9952446.
^ Davidson, Sandra E.; Smith, Jennifer J.; Helliwell, Chris A.; Poole, Andrew T.; Reid, James B. (2004). "The Pea Gene LH Encodes ent-Kaurene Oxidase". Plant Physiology. 134 (3): 1123–1134. doi:10.1104/pp.103.032706. PMC 389936. PMID 14988475.

Ent-kaurene+oxidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Cytochromes, oxygenases: cytochrome P450 (Most belong to EC 1.14)
CYP1	A1 A2 A5 B1
CYP2	A6 A7 A13 B6 C8 C9 C18 C19 D6 E1 F1 J2 R1 S1 U1 W1
CYP3 (CYP3A)	A4 A5 A7 A37 A43
CYP4	A11 A22 B1 F2 F3 F8 F11 F12 F22 V2 X1 Z1
CYP5-20	CYP5 (A1) CYP6 (G1, M2) CYP7 (A1, B1) CYP8 (A1, B1) CYP9 CYP10 CYP11 (A1, A2, B1, B2, B3, C1) CYP12 CYP13 CYP14 CYP15 CYP16 CYP17 (A1) CYP18 CYP19 (A1) CYP20 (A1)
CYP21-49	CYP21 (A2) CYP22 (A1) CYP23 CYP24 (A1) CYP25 CYP26 (A1, B1, C1) CYP27 (A1, B1, C1) CYP28 (A1) CYP29 CYP35 (B1) CYP39 (A1) CYP46 (A1)
CYP51-69	CYP51 (A1, F1) CYP52 CYP53 A1 CYP55 A1 CYP56 A1 CYP61
CYP71-99	CYP71 (C1, C2, C3, C4, Z6, AJ4, AV1, BA1) CYP72A1 CYP73A1 CYP74 (D1) CYP75 (A1, B1) CYP76 (B6, M7) CYP79 (A1, A2, B1, B2, B3, D1, D2, D3, D4) CYP80 (A1, B1, G2) CYP81 (E1, E3, E7, E9) CYP88D6 CYP90C1 CYP93E1 CYP97C1 CYP99A3
CYP101-281	CYP101A1 CYP102A1 CYP105 A1 D7 CYP106A2 CYP107 A1 G1 CYP109 B1 E1 CYP111 CYP113A1 CYP119A1 CYP123 CYP125A1 CYP139 CYP147 CYP152 A1 B1 CYP154C3 CYP158A CYP161C CYP170 A1 B1 CYP176A1 CYP183A CYP197 CYP199A2 CYP255A
CYP301-499	CYP303A1 CYP305 M2 Halloween genes ( CYP302A1, CYP306A1, CYP307A1, CYP307A2, CYP314A1, CYP315A1) CYP318A1
CYP501-699	CYP503 CYP504B1
CYP701-999	CYP704B22 CYP710 CYP720A1

This enzyme-related article is a stub. You can help Wikipedia by expanding it.

Hidden category: